Study on the biodegradation of crude oil by free and. Bacteria capable of denitrification are spread among phylogenetically diverse groups. The observation that the oceans are not covered with an oily layer is a testimony to the activity of the hydrocarbon. Elghazawani2 and ahmed issa3 1microbiology laboratory, botany department, omar almukhtar university, elbeida libya 2inspection and corrosion section, sarir. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38. Characterization of diesel degrading bacterial species. Pdf isolation and characterization of plastic degrading. Isolation and identification of petroleum hydrocarbon.
Isolation and identification of high efficient oil. Isolation of hydrocarbon degrading bacteria from soils. This was incubated in dark at 28 32 o c for five days. Isolation and identification of hydrocarbon degrading. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. Isolation, identification and characterization of oil degrading bacteria isolated 431 biochemical characterization of selected microorganisms. Isolation and characterisation of crude oil sludge degrading bacteria. Isolation and characterization of cholesterol degrading bacteria from soap and vegetable oil industrial waste s saranya1, s shekinah1, t rajagopal2, j vijayakumar2 and p ponmanickam1 1department of microbiology and 2department of biotechnology, ayya nadar janaki ammal college autonomous, sivakasi 626 124, india. Alsaleh department of biological science, kuwait university, kuwait abstract the planning of cities must insure a balance between human consumption and. The presence of oildegrading bacteria is a prerequisite for oil degradation to occur. An experimental study was undertaken to assess the efficiency of pseudomonas aeruginosa, bacillus subtilis, and acinetobacter lwoffi isolated from petroleum contaminated water and soil samples to degrade crude oil, separately and in a mixed bacterial consortium.
For these facultative hydrocarbon degraders the occasional supertanker oil spill forms. Isolation and characterisation of crude oil sludge. Adeleke1,3,4 background high demand for petroleum products all over the world has led to an increase in crude. Extraction and characterization of oil degrading bacteria. Isolation, identification and characterization of oil.
Isolation and characterization of hydrocarbondegrading bacteria. Arctic marine potential of microbial oil degradation aarhus universitet. Meet the microbes eating the gulf oil spill slide show. For the treatment of waste and waste water the use of the oil degrading bacteria has been strongly applied in controlled systems hazen, et, al, 2010. Native bacteria were isolated and screened based on their potential in reduction of total petroleum hydrocarbons, hemolysis, oil spreading technique and gas. Isolation of crude oil degrading marine bacteria and. Gene cloning, li, oil degrading, pseudomonas aeruginosa introduction pseudomonas aeruginosa is an opportunistic bacterium that lives in soil, water, and even in environments. Isolation and identification of petroleum hydrocarbon degrading bacteria from oil contaminated soil samples. Biodegradation of fats, oils, and greases in collection. Isolation and characterization of cyanide degrading.
Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with. Isolation and characterization of cholesterol degrading. Isolation and identification of oil degrading bacteria. Twentyfive crudeoildegrading bacteria were isolated from oilcontaminated sites in the persian gulf and the caspian sea. Pure cultures from the dieselenriched media were isolated by plating 1. Various traits, such as hemolytic activity, oil resolving, oil spreading, and oil emulsifying capability were used to screen the isolates.
They are called hydrocarbonoclastic bacteria and play a key role in the removal of hydrocarbons from polluted environments harayama et al. Reconstructing metabolic pathways of hydrocarbondegrading. Characterization of crude oildegrading bacteria in a crude oilcontaminated and uncontaminated site in kuwait a. This was evident as no zone of clearance was observed in the blood agar plates.
Several bacteria are even known to feed exclusively on hydrocarbons yakimov et al. Studies on crude oil degrading bacteria isolated from. Pdf on jan 1, 20, amit pandey and others published isolation of oil degrading bacteria from oil contaminated soil and expression of oil degrading genes in non oil degrading bacteria find. Isolation and characterization of biosurfactant producing. These isolates were purified from soil samples on the basis of their colony morphology, texture, growth, temperature and hydrocarbon utilization ability. The purpose of this study was to ascertain if the predominant species of oildegrading bacteria in salt marshes in barataria bay have changed since the spill in 2010 and to determine how well the indigenous hydrocarbondegrading bacteria are able to degrade crude oil. Hydrocarbons, enrichment culture technique, oil degrading bacteria, gravimetric analysis, biochemical tests. For the isolation of diesel degrading bacteria 31 soil samples were analyzed. Environmental studies on the microbial degradation of oil. Isolation and characterization of vegetable oil degrading bacteria article pdf available in journal of pure and applied microbiology 52. Of the 20 isolates, ram03, ram06, ram, and ram17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. Department of biochemistry and molecular biology, school of life sciences, pondicherry university, puducherry, india a r t i c l e i n f o article history. Researchers at the university of georgia found that chemical dispersants meant to help oilmunching microbes break down oil may have the opposite effect. Abstract the role of plasmidborne genes in the biodegradation of chevron escravos crude oil by bacteria was determined.
Isolation, purification and characterization of diesel degrading bacterial species. A newly discovered type of oileating microbe suddenly is flourishing in the gulf of mexico and gobbling up the bp spill at a much faster rate than expected, scientists reported tuesday. Meet the microbes eating the gulf oil spill slide show these microscopic life forms are blooming as a result of the oil spilled into the gulf of mexico from the macondo 252 deepsea well. The modified polyelectrolytemagnetite nanocoating was. A total of 1 strains of hydrocarbon degrading bacteria were isolated from the surface sediment samples, during the period between july 2009 june 2010, slightly predominated by gramnegative bacteria. Degradation of poly aromatic fractions of crude oil and detection of catabolic genes in hydrocarbondegrading bacteria isolated from. The report is available in electronic format pdf at. Using physiological and biochemical identification, electron microscopy, and 16s rdna sequence analysis, strain sq was identified as advenella kashmirensis,sy was identified as achromobacter. International journal of ag riculture, environment and bioresear ch. Biodegradation of fats, oils, and greases in collection systems the role of bioaugmentation. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated. Twentythree hydrocarbondegrading bacteria strains were isolated from gas station leakingcontaminated groundwater located in the southern. Kerry evans remember the deepwater horizon oil spill. Capillary gas chromatography was used for testing the effect of those bacterial species on the biodegradation of crude oil.
Isolation and characterization of polythene degrading bacteria. A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. In addition, the presence of the catabolic gene, catechol2,3dioxygenase was detected in the bacteria isolates as well as in evolutionary classifications based on phylogeny. In recent years the microbial biodegradation of pollutants is a sustainable ways to clean up. Extraction and characterization of oil degrading bacteria article pdf available in journal of applied sciences 610 october 2006 with 2,247 reads how we measure reads. Isolation and identification of oil degrading bacteria from oil sludge. Culture media to screen petroleum degrading bacteria. Isolation, characterization and identification of diesel engine oil degrading bacteria from garage soil and comparison of their bioremediation potential teli nikhil 1, verma deepa 2, gavankar rohan 1 and bhalerao satish 3 1department of biotechnology, viva college, virar w, maharashtra, india.
Supplementary tables 1,2, figures 16 and references. Characterization of crude oil degrading bacteria isolated. Isolation and characterization of new marine oildegrading. The current study aims to isolate oil degrading bacteria from soil and to test the degrading ability of the isolated bacteria on different types of oils. The contaminated soils were located in tehran and isfahan oil refineries, iran. Isolation of crude oil degrading marine bacteria and assessment for biosurfactant production 4 of 7 positive for production of biosurfactant and emulsification of toluene table 2. Isolation and characterisation of crude oil sludge degrading bacteria linda u. The oil degrading bacteria and other microorganisms degraded the chemical substance this process is.
Isolation and characterization of crude oil degrading. Isolation and characterization of crudeoildegrading. Several studies have reported on the catabolic abilities of microorganisms such as fungi, bacteria and algae to degrade petroleum hydrocarbons. Conclusions the bacteria isolated in this study are potential agents for the bioremediation of crude oil sludge. The deepwater horizon spill spewed nearly 750 million liters of oil into the. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Characterization of crude oildegrading bacteria in a. Conditions regarding the ph, maximum concentration of cyanide, dry weight of microbes and presence of ammonia of the simulated effluent were optimized favoring the growth of cyanide degrading bacteria. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons e. Fifteen crude oil degrading bacteria were isolated from oil contaminated sites in the persian gulf at khorramshahr provenance. Oil dispersants hinder oildegrading bacteria microbiology. Mohite, ruby e jalgaonwala, shradha pawar, ankush morankar department of biotechnology, moolaji jaitha college, jalgaon425001, ms india abstract.
In this experiment, 25 oil degrading bacteria strains were isolated from the beach near dalian bay. Atlas and bartha, 1992 was transferred to each flask and sterilized. Environmental studies on the microbial degradation of oil hydrocarbons and its application in lebanese oil polluted coastal and marine ecosystem. Identification and cloning of oil degrading genes of. I want to screen petroleum degrading bacteria from soil. Isolation and characterization of plastic degrading bacteria. Three distinct groups of bacteria were isolated which are able to degrade cyanide present in the simulated media.
For several years scientists have pursued genetic modifications to enhance the ability of various types of engineered bacteria to degrade oil spills, whether on land or in sea. Bacterial biodegradation of crude oil using local isolates. Interestingly, the proportion of alkane degrading isolates was lower. Isolation, characterization and identification of diesel.
Characterization of dioxygenases and biosurfactants. A smart dress for oildegrading bacteria sciencedaily. Isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel article in journal of basic microbiology 426. Isolation and characterization of phenol degrading bacteria from oil contaminated soil bhavna v. In the present investigation, molecular methods amplified ribosomal dna restriction analysis ardra and partial 16s rdna gene sequencing were used to determine the genetic diversity of. Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria santhakumar muthukamalam, sivalingam sivagangavathi, dharmapal dhrishya, sadras sudha rani. Oil degrading bacteria are considered as the dominant hydrocarbon which helps in degrading the aquatic systems such as oceans. Isolation and characterization of bacteria from crude. The isolation of bacteria was done on nutrient agar, nutrient broth 32 and mineral salt medium msm, and ir spectroscopy was used to observe the degradation of 33 diesel. These bacterias are capable of diverse metabolic pathways which enable them to utilize most recalcitrant petroleum hydrocarbons that are not present in fungi. The aim of this work was to isolate and characterize bacteria from two crude oilcontaminated soils which were capable of producing biosurfactant and degrade oil. Oildegrading bacteria for applications in oil wells and. Isolation and characterization of hydrocarbondegrading.
Determination of predominant species of oildegrading. Pdf study of oil degrading bacteria isolated from oil. In recent years a study was undertaken to isolate crudeoil degrading bacteria from petroleumpolluted soil collected from oil fields in bahrain and kuwait and to assess their biodegradation potential. New microbe found eating gulf oil spill disaster in the. Reconstructing metabolic pathways of hydrocarbondegrading bacteria from the deepwater horizon. Biosurfactants production by the isolated bacteria was also investigated. Isolation and characterization of vegetable oil degrading. Several bacteria are even known to feed exclusively on hydrocarbons 39.
The extraction of crude oil from reservoirs has been shown to be a rather inefficient process. These bacteria were screened with two important factors such as growth rate on crude oil and hydrocarbon biodegradation, and then three strains were selected from 15 isolated strains for further study. Dispersants set the stage for oildegrading bacteria. Studies on crude oil degrading bacteria isolated from libyan desert farag m. Isolation and characterization of new marine oildegrading bacteria romanian biotechnological letters, vol. Biosurfactant produced by none of the isolates had hemolytic properties. Functional genetic diversity and culturability of petroleum. On the other hand, some bacterial species are highly specialized in degrading hydrocarbons. A brief survey of the history, methodology and microorganisms.
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